For growing need of cell disruption, nano particle production, liposome, emulsion and dispersion for Pharmaceuticals, Biopharmaceuticals and Biotechnological products we provide technologically advance High Pressure Homogenizer (HPH) from R&D scale to production scale. For specific inquiry please contact with Bio-Xin and fill the QUESTIONNAIRES for the selection of appropriate HPH for your application. Click here to download the Application Questionnaire.
For growing need of cell disruption, nano particle production, liposome, emulsion and dispersion for Pharmaceuticals, Biopharmaceuticals and Biotechnological products we provide technologically advance High Pressure Homogenizer (HPH) from R&D scale to production scale. For specific inquiry please contact with Bio-Xin and fill the QUESTIONNAIRES for the selection of appropriate HPH for your application. Click here to download the Application Questionnaire.
Cell Rupture:
| Strain: | Schizosaccharomyces pombe |
| Machine: | EmulsiFlex-C160 |
| Pressure: | 28000psi/193MPa |
| Sample | cfu/ml | % viable | % NOT viable |
| control | 5.1 x 107 | ~100 | ~0 |
| 1 pass | 5.0 x 103 | 0.01 | 99.99 |
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| A control sample of unruptured Schizosaccharomyces pombe yeast. | Schizosaccharomyces pombe after one pass at 28000 psi/193MPa through the EmulsiFlex-C160. Only cell fragments remain. |
| Strain: | E. coli (BL21) |
| Machine: | EmulsiFlex-C160 |
| Pressure: | 20000psi/138MPa |
| Sample | cfu/ml | % viable | % NOT viable |
| control | 4.06 x 109 | ~100 | ~0 |
| 1 pass | 1.77 x 107 | 0.44 | 99.56 |
| 2 passes | 1.70 x 106 | 0.04 | 99.96 |
| 3 passes | 2.40 x 104 | 5.9 x 10-4 | ~100 |
A whole range of dispersions can be run through EmulsiFlex high pressure homogenizers to reduce particle size as well as make the size distribution uniform. EmulsiFlex homogenizers are involved in the processing of dispersions such as drug dispersions, herb extracts, industrial coatings and pigments.
Shown is a sample of a dry crystalline (amorphous) powder run through an EmulsiFlex homogenizer at 25000psi/173MPa.
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| An unprocessed dispersion with a starting size range of 10-100microns (600X magnification). | Processed sample of uniform, 1micron particles (100X magnification). |
AVESTIN®'s high pressure homogenizers are used all over the world for liposome research and production. Only EmulsiFlex homogenizers offer the combination of high pressure homogenization, high pressure membrane filtration or a combined, homogenization/filtration process.
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| Liposomes prepared using the EmulsiFlex high pressure homogenizer, extruder or both in combination. Combined homogenization yields 113.7nm in one pass and 89.9nm in two passes. | An example of uniform, unilamellar liposomes. |
Oil in Water Emulsion
| Sample | Pressure (psi) | Diameter (nm) |
| Unprocessed | - | 656 |
| 1 Pass | 16,000 | 77 |
| 3 Passes | 16,000 | 59 |
| 5 Passes | 16,000 | 42 |
Pharmaceutical Emulsion
| Sample | Homogenizing Pressure (psi) | Mean Droplet Diameter (nm) |
| Unprocessed | - | 3,500 |
| 1 Pass | 20,000 | 590 |
| 3 Passes | 20,000 | 185 |
| 5 Passes | 20,000 | 140 |
 | Pharmaceutical drug emulsion 'A' before processing. The starting particle size is large (5-10microns) and the distribution wide. |
 | Pharmaceutical emulsion 'A' after processing at 18000psi/124MPa. Note the dramatic reduction in size (52nm) and the narrower distribution. |
